Rat IL-1β Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$450.00
Catalog Number : BGK5BKB0

description

IL-1b is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1a and IL-1b bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities, including the stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity, and, the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1b is a secreted cytokine, IL-1a is predominantly a cell-associated cytokine.

The BioGems Rat IL-1β Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-1β with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-1β. It contains E. coli-expressed recombinant IL-1β. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-1β. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1pg/ml
Range:
31.2pg/ml-2000pg/ml
Specificity:
Natural and recombinant Rat IL-1β
Uniprot:
Q5BKB0
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Rat IL-1β Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Rat IL-1β Standard

2

10 ng vial

Rat IL-1β Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

105

756

1359

112

688

1452

Standard Deviation

3.99

34.78

66.59

5.712

37.84

92.93

Consistency (%CV)

3.8

4.6

4.9

5.1

5.5

6.4

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