Murine IL-7 Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$440.00
Catalog Number : BGK10168

description

IL-7 is a hematopoietic growth factor that primarily affects early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors, such as ConA and IL-2.

The BioGems Murine IL-7 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-7 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-7. It contains E. coli-expressed recombinant IL-7. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-7. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1pg/ml
Range:
15.6pg/ml-1000pg/ml
Specificity:
Natural and recombinant Murine IL-7
Uniprot:
P10168
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Murine IL-7 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine IL-7 Standard

2

10 ng vial

Murine IL-7 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

47.8

376

755

52.5

388

746

Standard Deviation

2.49

13.16

35.49

3.31

32.59

40.3

Consistency (%CV)

5.2

3.5

4.7

6.3

8.4

5.4

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