Murine IL-6 Pre-Coated ELISA Kit
description
IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R α and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells.
The BioGems Murine IL-6 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine IL-6 with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for IL-6. It contains E. coli-expressed recombinant Murine IL-6. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-6. The kit is analytically validated with ready to use reagents.
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<1 pg/ml
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Range:
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15.6 pg/mL- 1,000 pg/mL
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Specificity:
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Natural and recombinant Murine IL-6
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Uniprot:
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P08505
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
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Description |
Quantity |
Volume |
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Anti-Murine IL-6 Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
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Murine IL-6 Standard |
2 |
10 ng vial |
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Murine IL-6 Biotinylated antibody (100x) |
1 |
130 µL |
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Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
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Sample Diluent |
1 |
30mL |
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Antibody Diluent |
1 |
12mL |
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Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
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Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
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Color Developing Reagent |
1 |
10mL |
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Stop Solution |
1 |
10mL |
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Plate Sealers |
4 |
Each |
Reproducibility
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Intra-Assay |
Inter-Assay |
||||
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Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
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n= |
16 |
16 |
16 |
24 |
24 |
24 |
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Mean Measured Concentration (pg/mL) |
97.5 |
359 |
654 |
102 |
437 |
716 |
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Standard Deviation |
6.24 |
16.16 |
31.39 |
7.65 |
25.78 |
37.95 |
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Consistency (%CV) |
6.4 |
4.5 |
4.8 |
7.5 |
5.9 |
5.3 |