Murine IL-3 Pre-Coated ELISA Kit
description
The BioGems Murine IL-3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-3. It contains E. coli-expressed recombinant IL-3. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-3. The kit is analytically validated with ready to use reagents.
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<1pg/ml
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Range:
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7.8pg/ml-500pg/ml
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Specificity:
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Natural and recombinant Murine IL-3
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Uniprot:
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P01586
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description | Quantity | Volume |
Anti-Murine IL-3 Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells |
Murine IL-3 Standard | 2 | 10 ng vial |
Murine IL-3 Biotinylated antibody (100x) | 1 | 130 µL |
Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL |
Sample Diluent | 1 | 30mL |
Antibody Diluent | 1 | 12mL |
Avidin-Biotin-Peroxidase Diluent | 1 | 12mL |
Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack |
Color Developing Reagent | 1 | 10mL |
Stop Solution | 1 | 10mL |
Plate Sealers | 4 | Each |
Reproducibility
| Intra-Assay | Inter-Assay | ||||
Sample ID | 1 | 2 | 3 | 1 | 2 | 3 |
n= | 16 | 16 | 16 | 24 | 24 | 24 |
Mean Measured Concentration (pg/mL) | 32.6 | 156 | 364 | 43.7 | 164 | 348 |
Standard Deviation | 1.793 | 9.516 | 23.66 | 2.75 | 11.81 | 26.1 |
Consistency (%CV) | 5.5 | 6.1 | 6.5 | 6.3 | 7.2 | 7.5 |