Murine IL-3 Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$405.00
Catalog Number : BGK01586

description

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines.

The BioGems Murine IL-3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-3. It contains E. coli-expressed recombinant IL-3. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-3. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1pg/ml
Range:
7.8pg/ml-500pg/ml
Specificity:
Natural and recombinant Murine IL-3
Uniprot:
P01586
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Murine IL-3 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine IL-3 Standard

2

10 ng vial

Murine IL-3 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

32.6

156

364

43.7

164

348

Standard Deviation

1.793

9.516

23.66

2.75

11.81

26.1

Consistency (%CV)

5.5

6.1

6.5

6.3

7.2

7.5

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