Murine IL-2 Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$295.00
Catalog Number : BGK04351

description

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation.  IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response.  IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.

The BioGems Murine IL-2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine IL-2 with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for IL-2. It contains E. coli-expressed recombinant Murine IL-2. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-2. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1 pg/ml
Range:
15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Murine IL-2
Uniprot:
P04351
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Murine IL-2 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine IL-2 Standard

2

10 ng vial

Murine IL-2 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

81

215

590

86

193

575

Standard Deviation

4.536

11.61

25.37

5.246

13.32

29.9

Consistency (%CV)

5.6

5.4

4.3

6.1

6.9

5.2

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