Murine IL-17A Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$340.00
Catalog Number : BGK62386

description

The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136 amino acid chains that are secreted by activated T-cells, which act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules.  Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region, but differ from one another in their N-terminal regions and in their distinct biological roles.  The six known members of this family, IL-17A through IL-17F, are secreted as homodimers.  IL-17A exhibits cross-species bioactivity between human and murine cells.

The BioGems Murine IL-17A Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine IL-17A with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for IL-17A. It contains E. coli-expressed recombinant Murine IL-17A. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-17A. The kit is analytically validated with ready to use reagents.

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Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1 pg/ml
Range:
15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Murine IL-17A
Uniprot:
Q62386
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Murine IL-17A Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine IL-17A Standard

2

10 ng vial

Murine IL-17A Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

83

372

623

103

418

737

Standard Deviation

3.82

20.46

37.38

5.46

25.92

50.12

Consistency (%CV)

4.6

5.5

6

5.3

6.2

6.8

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