Murine IL-17A Pre-Coated ELISA Kit
description
The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136 amino acid chains that are secreted by activated T-cells, which act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region, but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells.
The BioGems Murine IL-17A Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine IL-17A with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for IL-17A. It contains E. coli-expressed recombinant Murine IL-17A. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-17A. The kit is analytically validated with ready to use reagents.
Product Details
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
Sensitivity:
|
<1 pg/ml
|
Range:
|
15.6 pg/mL- 1,000 pg/mL
|
Specificity:
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Natural and recombinant Murine IL-17A
|
Uniprot:
|
Q62386
|
Interference:
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No detectable cross-reactivity with other relevant proteins
|
Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description |
Quantity |
Volume |
Anti-Murine IL-17A Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Murine IL-17A Standard |
2 |
10 ng vial |
Murine IL-17A Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility
|
Intra-Assay |
Inter-Assay |
||||
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
83 |
372 |
623 |
103 |
418 |
737 |
Standard Deviation |
3.82 |
20.46 |
37.38 |
5.46 |
25.92 |
50.12 |
Consistency (%CV) |
4.6 |
5.5 |
6 |
5.3 |
6.2 |
6.8 |