Murine IL-13 Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$485.00
Catalog Number : BGK20109

description

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma.

The BioGems Murine IL-13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-13 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-13. It contains E. coli-expressed recombinant IL-13. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-13. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<2pg/ml
Range:
15.6pg/ml-1000pg/ml
Specificity:
Natural and recombinant Murine IL-13
Uniprot:
P20109
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Murine IL-13 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine IL-13 Standard

2

10 ng vial

Murine IL-13 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

112

355

628

149

417

674

Standard Deviation

6.16

21.3

29.52

9.24

29.61

39.1

Consistency (%CV)

5.5

6

4.7

6.2

7.1

5.8

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