Murine IL-1β Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$340.00
Catalog Number : BGK10749

description

IL-1β is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells.  Both IL-1α and IL-1β bind to the same receptor and have similar, if not identical, biological properties.  These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells.  However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine.

The BioGems Murine IL-1β Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine IL-1β with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for IL-1β. It contains E. coli-expressed recombinant Murine IL-1β. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-1β. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1 pg/ml
Range:
12.5 pg/mL- 800 pg/mL
Specificity:
Natural and recombinant Murine IL-1β
Uniprot:
P10749
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Murine IL-1β Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine IL-1β Standard

2

10 ng vial

Murine IL-1β Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

86

376

587

79

346

558

Standard Deviation

6.97

19.55

24.07

7.03

25.6

37.94

Consistency (%CV)

8.1

5.2

4.1

8.9

7.4

6.8

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