Murine IFN-γ Pre-Coated ELISA Kit

Grouped product items
Size Price Qty
1 Kit
$340.00
Catalog Number : BGK01580

description

IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes, as well as activated NK cells.  IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins.  This promotes the presentation of antigen to T-helper (CD4+) cells.  IFN-γ signaling in antigen-presenting, cells and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response.  Additionally, IFN-γ stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils.  Human IFN-γ is species-specific and is biologically active only in human and primate cells.

The BioGems Murine IFN-γ Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine IFN-γ with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for IFN-γ. It contains E. coli-expressed recombinant Murine IFN-γ. The detection antibody is a biotinylated goat polyclonal antibody specific for IFN-γ. The kit is analytically validated with ready to use reagents.

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<5 pg/ml
Range:
31.2 pg/mL- 2,000 pg/mL
Specificity:
Natural and recombinant Murine IFN-γ
Uniprot:
P01580
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Murine IFN-γ Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine IFN-γ Standard

2

10 ng vial

Murine IFN-γ Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

305

782

1367

350

812

1494

Standard Deviation

11.59

35.19

69.72

19.6

55.22

109.1

Consistency (%CV)

3.8

4.5

5.1

5.6

6.8

7.3

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