Murine IFN-γ Pre-Coated ELISA Kit
description
IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes, as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting, cells and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ is species-specific and is biologically active only in human and primate cells.
The BioGems Murine IFN-γ Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine IFN-γ with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for IFN-γ. It contains E. coli-expressed recombinant Murine IFN-γ. The detection antibody is a biotinylated goat polyclonal antibody specific for IFN-γ. The kit is analytically validated with ready to use reagents.
Product Details
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
Sensitivity:
|
<5 pg/ml
|
Range:
|
31.2 pg/mL- 2,000 pg/mL
|
Specificity:
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Natural and recombinant Murine IFN-γ
|
Uniprot:
|
P01580
|
Interference:
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No detectable cross-reactivity with other relevant proteins
|
Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description |
Quantity |
Volume |
Anti-Murine IFN-γ Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Murine IFN-γ Standard |
2 |
10 ng vial |
Murine IFN-γ Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility
|
Intra-Assay |
Inter-Assay |
||||
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
305 |
782 |
1367 |
350 |
812 |
1494 |
Standard Deviation |
11.59 |
35.19 |
69.72 |
19.6 |
55.22 |
109.1 |
Consistency (%CV) |
3.8 |
4.5 |
5.1 |
5.6 |
6.8 |
7.3 |