Murine HGF Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$410.00
Catalog Number : BGK08048

description

HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include the induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Murine HGF is expressed as a linear, polypeptide-precursor glycoprotein containing 696 amino acid residues. Proteolytic processing of this precursor generates the biologically active heterodimeric form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held together by a single disulfide bond resulting in formation of a biologically active heterodimer.

The BioGems Murine HGF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure HGF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for HGF. It contains NS0-expressed recombinant HGF. The detection antibody is a biotinylated goat polyclonal antibody specific for HGF. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
156pg/ml-10000pg/ml
Specificity:
Natural and recombinant Murine HGF
Uniprot:
Q08048
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Murine HGF Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine HGF Standard

2

10 ng vial

Murine HGF Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

212

1030

4401

288

958

3852

Standard Deviation

13.14

56.65

246.45

20.73

58.43

231.12

Consistency (%CV)

6.2

5.5

5.6

7.2

6.1

6

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