Human VEGF Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$365.00
Catalog Number : BGK15692

description

VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. VEGF signals through three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.

The BioGems Human VEGF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human VEGF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for VEGF. It contains sf21-expressed recombinant Human VEGF. The detection antibody is a biotinylated goat polyclonal antibody specific for VEGF. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1 pg/ml
Range:
31.2 pg/mL- 2,000 pg/mL
Specificity:
Natural and recombinant Human VEGF
Uniprot:
P15692
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human VEGF Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human VEGF Standard

2

10 ng vial

Human VEGF Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

146

650

1173

242

567

1084

Standard Deviation

6.57

24.7

63.34

12.1

27.78

70.46

Consistency (%CV)

4.5

3.8

5.4

5

4.9

6.5

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