Human TLR-3 Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$460.00
Catalog Number : BGK6Y0F1

description

TLR-3 is a single-pass type I receptor that binds to and signals the presence of microbial pathogens and double stranded RNA (dsRNA) viruses. Signaling through TLR-3 can promote the NF-κB pathway to initiate innate and adaptive immune responses to bacterial and viral infections, as well as the p53 pathway to trigger apoptosis in cells infected with dsRNA viruses. TLR-3 belongs to a family of structurally-related toll-like receptors (TLRs) containing an N-terminal domain rich in leucine repeats, and a C-terminal intracellular Toll/interleukin (IL)-1 (TIL) domain. TLR-3 is expressed primarily in dendritic cells of the placenta and pancreas where it can reside on both sides of the plasma membrane, and in the endosomal compartment of the cells.

The BioGems Human TLR-3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure TLR-3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TLR-3. It contains NS0-expressed recombinant TLR-3. The detection antibody is a biotinylated goat polyclonal antibody specific for TLR-3. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
156pg/ml-10,000pg/ml
Specificity:
Natural and recombinant Human TLR-3
Uniprot:
E6Y0F1
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human TLR-3 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human TLR-3 Standard

2

10 ng vial

Human TLR-3 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

824

2625

5720

987

3013

6325

Standard Deviation

37.08

147

366.1

53.3

213.9

493.3

Consistency (%CV)

4.5

5.6

6.4

5.4

7.1

7.8

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