Human TGF-α Pre-Coated ELISA Kit
description
The BioGems Human TGF-α Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure TGF-α with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TGF-α. It contains E. coli-expressed recombinant TGF-α. The detection antibody is a biotinylated goat polyclonal antibody specific for TGF-α. The kit is analytically validated with ready to use reagents.
Product Details
|
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
|
Sensitivity:
|
<1pg/ml
|
|
Range:
|
15.6pg/ml-1000pg/ml
|
|
Specificity:
|
Natural and recombinant Human TGF-α
|
|
Uniprot:
|
P01135
|
|
Interference:
|
No detectable cross-reactivity with other relevant proteins
|
|
Storage Conditions:
|
Store the unopened product at 2 - 8 °C.
|
Components
Description | Quantity | Volume |
Anti-Human TGF-α Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells |
Human TGF-α Standard | 2 | 10 ng vial |
Human TGF-α Biotinylated antibody (100x) | 1 | 130 µL |
Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL |
Sample Diluent | 1 | 30mL |
Antibody Diluent | 1 | 12mL |
Avidin-Biotin-Peroxidase Diluent | 1 | 12mL |
Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack |
Color Developing Reagent | 1 | 10mL |
Stop Solution | 1 | 10mL |
Plate Sealers | 4 | Each |
Reproducibility
| Intra-Assay | Inter-Assay | ||||
Sample ID | 1 | 2 | 3 | 1 | 2 | 3 |
n= | 16 | 16 | 16 | 24 | 24 | 24 |
Mean Measured Concentration (pg/mL) | 118 | 225 | 516 | 126 | 238 | 509 |
Standard Deviation | 5.43 | 12.83 | 16.5 | 8.95 | 14.28 | 27 |
Consistency (%CV) | 4.6 | 5.7 | 3.2 | 7.1 | 6 | 5.3 |