Human TGF-α Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$435.00
Catalog Number : BGK01135

description

TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-α induces transformation anchorage independence in cultured cells.

The BioGems Human TGF-α Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure TGF-α with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TGF-α. It contains E. coli-expressed recombinant TGF-α. The detection antibody is a biotinylated goat polyclonal antibody specific for TGF-α. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1pg/ml
Range:
15.6pg/ml-1000pg/ml
Specificity:
Natural and recombinant Human TGF-α
Uniprot:
P01135
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human TGF-α Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human TGF-α Standard

2

10 ng vial

Human TGF-α Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

118

225

516

126

238

509

Standard Deviation

5.43

12.83

16.5

8.95

14.28

27

Consistency (%CV)

4.6

5.7

3.2

7.1

6

5.3

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