Human TGF-β1 Pre-Coated ELISA Kit

Grouped product items
Size Price Qty
1 Kit
$410.00
Catalog Number : BGK01137

description

The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors.

The BioGems Human TGF-β1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TGF-β1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TGF-β1. It contains CHO-expressed recombinant Human TGF-β1. The detection antibody is a biotinylated goat polyclonal antibody specific for TGF-β1. The kit is analytically validated with ready to use reagents.

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1 pg/ml
Range:
15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Human TGF-β1
Uniprot:
P01137
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human TGF-β1 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human TGF-β1 Standard

2

10 ng vial

Human TGF-β1 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

347

1638

2586

511

1825

3120

Standard Deviation

13.88

85.18

163

29.64

100.4

246.5

Consistency (%CV)

4

5.2

6.3

5.8

5.5

7.9

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