Human TFF-3 Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$435.00
Catalog Number : BGK07654

description

The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are secreted in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF-3 is expressed by goblet cells and in the uterus, and has also been shown to be expressed in certain cancers, including colorectal, hepatocellular, and in biliary tumors. TFF3 may be useful as a molecular marker for certain types of cancer, but its role, if any, in tumorigenesis is unknown. TFF3 also promotes airway epithelial cell migration and differentiation.

The BioGems Human TFF-3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure TFF-3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TFF-3. It contains E. coli-expressed recombinant TFF-3. The detection antibody is a biotinylated goat polyclonal antibody specific for TFF-3. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
62.5pg/ml-4000pg/ml
Specificity:
Natural and recombinant Human TFF-3 Pre-Coated ELISA Kit
Uniprot:
Q07654
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human TFF-3 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human TFF-3 Standard

2

10 ng vial

Human TFF-3 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

121

446

1356

103

414

1877

Standard Deviation

5.57

23.64

80

7.31

29.8

86.34

Consistency (%CV)

4.6

5.3

5.9

7.1

7.2

4.6

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