Human PD-L1/B7-H1 Pre-Coated ELISA Kit

Grouped product items
Size Price Qty
1 Kit
$410.00
Catalog Number : BGK9NZQ7

description

Programmed death-ligand 1 (PD-L1) is a typ1 transmembrane glycoprotein also known as CD274 and was first characterized as an immune regulatory molecule, B7-H1. The PD-1 receptor is found on activated B cells, T cells, and myeloid cells and modulates activation and inhibition. The binding of PD-L1 to the PD-1 receptor on T cells delivers a signal that inhibits the TCR-mediated activation of IL-2 production and T cell proliferation. Research has determined that the upregulation of PD-L1 may allow cancers to evade the host immune system.   

The BioGems Human PD-L1/B7-H1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PD-L1/B7-H1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for B7-H1/PD-L1. It contains NS0-expressed recombinant Human PD-L1/B7-H1. The detection antibody is a biotinylated goat polyclonal antibody specific for PD-L1/B7-H1. The kit is analytically validated with ready to use reagents.

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<12 pg/ml
Range:
62.5 pg/mL- 4000 pg/mL
Specificity:
Natural and recombinant Human PD-L1/B7-H1
Uniprot:
Q9NZQ7
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human PD-L1/B7-H1 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human PD-L1/B7-H1 Standard

2

10 ng vial

Human PD-L1/B7-H1 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

432

1131

2274

487

1452

2635

Standard Deviation

20.74

44.11

122.80

35.55

90.02

176.55

Consistency (%CV)

4.8

3.9

5.4

7.3

6.2

6.7

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