Human PD-L1/B7-H1 Pre-Coated ELISA Kit
description
Programmed death-ligand 1 (PD-L1) is a typ1 transmembrane glycoprotein also known as CD274 and was first characterized as an immune regulatory molecule, B7-H1. The PD-1 receptor is found on activated B cells, T cells, and myeloid cells and modulates activation and inhibition. The binding of PD-L1 to the PD-1 receptor on T cells delivers a signal that inhibits the TCR-mediated activation of IL-2 production and T cell proliferation. Research has determined that the upregulation of PD-L1 may allow cancers to evade the host immune system.
The BioGems Human PD-L1/B7-H1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PD-L1/B7-H1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for B7-H1/PD-L1. It contains NS0-expressed recombinant Human PD-L1/B7-H1. The detection antibody is a biotinylated goat polyclonal antibody specific for PD-L1/B7-H1. The kit is analytically validated with ready to use reagents.
Product Details
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
Sensitivity:
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<12 pg/ml
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Range:
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62.5 pg/mL- 4000 pg/mL
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Specificity:
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Natural and recombinant Human PD-L1/B7-H1
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Uniprot:
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Q9NZQ7
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description |
Quantity |
Volume |
Anti-Human PD-L1/B7-H1 Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Human PD-L1/B7-H1 Standard |
2 |
10 ng vial |
Human PD-L1/B7-H1 Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility
|
Intra-Assay |
Inter-Assay |
||||
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
432 |
1131 |
2274 |
487 |
1452 |
2635 |
Standard Deviation |
20.74 |
44.11 |
122.80 |
35.55 |
90.02 |
176.55 |
Consistency (%CV) |
4.8 |
3.9 |
5.4 |
7.3 |
6.2 |
6.7 |