Human PAI-1 Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$480.00
Catalog Number : BGK05121

description

Plasminogen Activator Inhibitor-1 (PAI-1, Serpin E1) is a member of the serpin family of serine protease inhibitors, and is the primary inhibitor of urokinase and tissue plasminogen activator (tPA). PAI-1 is expressed predominantly in adipose, liver and vascular tissues, but is also produced by certain tumor cells. Elevated levels of PAI-1 are associated with obesity, diabetes and cardiovascular disease, and increased production of PAI-1 is induced by various obesity-related factors, such as TNFα, glucose, insulin, and very-low-density lipoprotein. The obesity-related elevation of PAI-1 levels, along with the consequential deficiency in plasminogen activators, can lead directly to increased risk of thrombosis and other coronary diseases. Accordingly, PAI-1 has been implicated as an important molecular link between obesity and coronary disease. PAI-1 can also specifically bind vitronectin (VTN) to form a stable active complex with an increased circulatory half-life relative to free PAI-1.

The BioGems Human PAI-1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure PAI-1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for PAI-1. It contains sf21-expressed recombinant PAI-1. The detection antibody is a biotinylated goat polyclonal antibody specific for PAI-1. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
156pg/ml-10,000pg/ml
Specificity:
Natural and recombinant Human PAI-1 Pre-Coated ELISA Kit
Uniprot:
P05121
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human PAI-1 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human PAI-1 Standard

2

10 ng vial

Human PAI-1 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

1539

3736

6234

1637

3914

7335

Standard Deviation

76.95

235.4

405.21

104.8

293.55

601.47

Consistency (%CV)

5

6.3

6.5

6.4

7.5

8.2

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