Human MCP-1 (CCL2) Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$370.00
Catalog Number : BGK13500

description

The MCP proteins are members of the CC chemokine family that signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross-reacts across species.

The BioGems Human MCP-1 (CCL2) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MCP-1 (CCL2) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MCP-1 (CCL2). It contains E. coli-expressed recombinant MCP-1 (CCL2). The detection antibody is a biotinylated goat polyclonal antibody specific for MCP-1 (CCL2). The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1pg/ml
Range:
15.6pg/ml-1000pg/ml
Specificity:
Natural and recombinant Human MCP-1 (CCL2)
Uniprot:
P13500
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human MCP-1 (CCL2) Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human MCP-1 (CCL2) Standard

2

10 ng vial

Human MCP-1 (CCL2) Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

73

362

663

93

397

695

Standard Deviation

2.7

17.74

34.5

5.022

24.22

51.43

Consistency (%CV)

3.7

4.9

5.2

5.4

6.1

7.4

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