Human IL-4 Pre-Coated ELISA Kit
description
IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.
The BioGems Human IL-4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL-4 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-4. It contains E. coli-expressed recombinant Human IL-4. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-4. The kit is analytically validated with ready to use reagents.
Product Details
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
Sensitivity:
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<1.5 pg/ml
|
Range:
|
15.6 pg/mL- 1,000 pg/mL
|
Specificity:
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Natural and recombinant Human IL-4
|
Uniprot:
|
P05112
|
Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description |
Quantity |
Volume |
Anti-Human IL-4 Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Human IL-4 Standard |
2 |
10 ng vial |
Human IL-4 Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility
|
Intra-Assay |
Inter-Assay |
||||
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
55.4 |
361 |
638 |
45.8 |
375 |
656 |
Standard Deviation |
2.5 |
14.8 |
37.64 |
2.977 |
24 |
46.58 |
Consistency (%CV) |
4.5 |
4.1 |
5.9 |
6.5 |
6.4 |
7.1 |