Human IL-13 Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$390.00
Catalog Number : BGK35225

description

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells.  Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites.  IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8.  IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in increased production of IgE.  Blocking of IL-13 activity inhibits the pathophysiology of asthma.  Human and murine IL-13 are cross-species reactive.

The BioGems Human IL-13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL-13 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-13. It contains E. coli-expressed recombinant Human IL-13. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-13. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10 pg/ml
Range:
15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Human IL-13
Uniprot:
P35225
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human IL-13 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human IL-13 Standard

2

10 ng vial

Human IL-13 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

98

340

497

115

354

512

Standard Deviation

4.02

10.88

19.38

8.28

19.82

29.7

Consistency (%CV)

4.1

3.2

3.9

7.2

5.6

5.8

© BioGems International, Inc. All rights reserved.