Human IL-12 (p70) Pre-Coated ELISA Kit
description
IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-γ-producing Th1 cells. Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).
The BioGems Human IL-12(p70) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL-12(p70) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-12(p70). It contains sf21-expressed recombinant Human IL-12(p70). The detection antibody is a biotinylated goat polyclonal antibody specific for IL-12(p70). The kit is analytically validated with ready to use reagents.
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<2 pg/ml
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Range:
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7.8 pg/mL- 500 pg/mL
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Specificity:
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Natural and recombinant Human IL-12(p70)
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Uniprot:
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P29459
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
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Description |
Quantity |
Volume |
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Anti-Human IL-12(p70) Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
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Human IL-12(p70) Standard |
2 |
10 ng vial |
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Human IL-12(p70) Biotinylated antibody (100x) |
1 |
130 µL |
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Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
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Sample Diluent |
1 |
30mL |
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Antibody Diluent |
1 |
12mL |
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Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
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Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
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Color Developing Reagent |
1 |
10mL |
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Stop Solution |
1 |
10mL |
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Plate Sealers |
4 |
Each |
Reproducibility
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|
Intra-Assay |
Inter-Assay |
||||
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Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
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n= |
16 |
16 |
16 |
24 |
24 |
24 |
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Mean Measured Concentration (pg/mL) |
44 |
127 |
283 |
67 |
158 |
322 |
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Standard Deviation |
2.16 |
7.24 |
17.83 |
3.89 |
9.48 |
23.2 |
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Consistency (%CV) |
4.9 |
5.7 |
6.3 |
5.8 |
6 |
7.2 |