Human IL-12 (p70) Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$345.00
Catalog Number : BGK29459

description

IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T cells.  It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells.  Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-γ-producing Th1 cells.  Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).

The BioGems Human IL-12(p70) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL-12(p70) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-12(p70). It contains sf21-expressed recombinant Human IL-12(p70). The detection antibody is a biotinylated goat polyclonal antibody specific for IL-12(p70). The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<2 pg/ml
Range:
7.8 pg/mL- 500 pg/mL
Specificity:
Natural and recombinant Human IL-12(p70)
Uniprot:
P29459
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human IL-12(p70) Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human IL-12(p70) Standard

2

10 ng vial

Human IL-12(p70) Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

44

127

283

67

158

322

Standard Deviation

2.16

7.24

17.83

3.89

9.48

23.2

Consistency (%CV)

4.9

5.7

6.3

5.8

6

7.2

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