Human HB-EGF Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$415.00
Catalog Number : BGK99075

description

HB-EGF is an EGF-related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells, and vascular SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF.

The BioGems Human HB-EGF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure HB-EGF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for HB-EGF. It contains sf21-expressed recombinant HB-EGF. The detection antibody is a biotinylated goat polyclonal antibody specific for HB-EGF. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
7.8pg/ml-500pg/ml
Specificity:
Natural and recombinant Human HB-EGF
Uniprot:
Q99075
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human HB-EGF Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human HB-EGF Standard

2

10 ng vial

Human HB-EGF Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

22.7

105

321

30.1

118

418

Standard Deviation

1.18

4.83

12.52

2.44

8.73

28.42

Consistency (%CV)

5.2

4.6

3.9

8.1

7.4

6.8

© BioGems International, Inc. All rights reserved.