Human FSTL1 Pre-Coated ELISA Kit
description
The BioGems Human FSTL1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure FSTL1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for FSTL1. It contains NS0-expressed recombinant FSTL1. The detection antibody is a biotinylated goat polyclonal antibody specific for FSTL1. The kit is analytically validated with ready to use reagents.
Product Details
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
Sensitivity:
|
<10pg/ml
|
Range:
|
312pg/ml-20,000pg/ml
|
Specificity:
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Natural and recombinant Human FSTL1
|
Uniprot:
|
Q12841
|
Interference:
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No detectable cross-reactivity with other relevant proteins
|
Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description |
Quantity |
Volume |
Anti-Human FSTL1 Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Human FSTL1 Standard |
2 |
20 ng vial |
Human FSTL1 Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility
| Intra-Assay | Inter-Assay | ||||
Sample ID | 1 | 2 | 3 | 1 | 2 | 3 |
n= | 16 | 16 | 16 | 24 | 24 | 24 |
Mean Measured Concentration (pg/mL) | 1.34 | 7.2 | 13.9 | 1.25 | 7.5 | 14.4 |
Standard Deviation | 0.056 | 0.382 | 0.89 | 0.094 | 0.48 | 1.123 |
Consistency (%CV) | 4.2 | 5.3 | 6.4 | 7.5 | 6.4 | 7.8 |