Human CTACK (CCL27) Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$445.00
Catalog Number : BGK9Y4X3

description

CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines.

The BioGems Human CTACK (CCL27) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure CTACK (CCL27) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for CTACK (CCL27). It contains E. coli-expressed recombinant CTACK (CCL27). The detection antibody is a biotinylated goat polyclonal antibody specific for CTACK (CCL27). The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
31.2pg/ml-2000pg/ml
Specificity:
Natural and recombinant Human CTACK (CCL27) Pre-Coated ELISA Kit
Uniprot:
Q9Y4X3
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human CTACK (CCL27) Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human CTACK (CCL27) Standard

2

10 ng vial

Human CTACK (CCL27) Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

352

794

1456

376

822

1570

Standard Deviation

14.78

30.97

77.17

31.58

64.94

111.47

Consistency (%CV)

4.2

3.9

5.3

8.4

7.9

7.1

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