Human BCA-1 (CXCL13) Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$400.00
Catalog Number : BGK43927

description

BCA-1/BLC, a CXC chemokine, is expressed in the liver, spleen, lymph nodes, appendix and stomach. It exerts its activities through its only receptor, CXCR5. BCA-1/BLC is a potent chemoattractant for B lymphocytes, and induces weak chemotactic response in T cells and macrophages. It manifests no activity on neutrophils and monocytes.

The BioGems Human BCA-1 (CXCL13) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure BCA-1 (CXCL13) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for BCA-1 (CXCL13). It contains E. coli-expressed recombinant BCA-1 (CXCL13). The detection antibody is a biotinylated goat polyclonal antibody specific for BCA-1 (CXCL13). The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1pg/ml
Range:
15.6pg/ml-1000pg/ml
Specificity:
Natural and recombinant Human BCA-1 (CXCL13) Pre-Coated ELISA Kit
Uniprot:
O43927
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human BCA-1 (CXCL13) Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human BCA-1 (CXCL13) Standard

2

10 ng vial

Human BCA-1 (CXCL13) Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

54.2

249

469

53.6

238

512

Standard Deviation

3.63

9.46

18.3

4.4

11.7

26.1

Consistency (%CV)

6.7

3.8

3.9

8.2

4.9

5.1

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