Recombinant laminin-511 (BG iMatrix-511)
description
BG iMatrix 511 is a chemically defined, animal-free, and xeno-free laminin-511 E8 fragment. Laminin-511 is well known to bind to integrin α6ß1, which is located on the cell surface and acts as a substrate for stem cell culture media for various cell types including ESCs and iPSCs. Recombinant laminin-511 E8 fragments are truncated, functional forms of laminin composed of the C-terminal regions of the alpha, beta, and gamma chains. Laminin-511 E8 (150 kDa) retains the full capability for binding to alpha 6 beta 1 integrin.
The 350ug size is supplied as 2x175ug vials. The 1050ug size is supplied as 6x175ug vials.
Review the Data Sheet and Product Brochure for more details.
Additional Information
Applications:
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Cell Culture
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Research Interest: | Stem Cell Research |
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Cell Type: | Stem Cells |
Application: | Cell Culture |
Application Notes:
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Formulation:
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0.5mg/mL in PBS(-).
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References:
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Taniguchi, Y., Ido, H., Sanzen, N., Hayashi, M., Sato-Nishiuchi, R., Futaki, S., & Sekiguchi, K. . The C-terminal region of laminin β chains modulates the integrin binding affinities of laminins. Journal of Biological Chemistry, 284(12), 7820-7831, (2009) Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., & Kawase, E. . Laminin E8 fragment support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nature communications, 3(1236), 1-10, (2012) Burridge, P. W., Matsa, E., Shukla, P., Lin, Z. C., Churko, J. M., Ebert, A. D., Lan, F., Diecke, S., Huber, B., Mordwinkin, N. M., Plews, J. R., Abilez, O. J., Cui, B., Gold, J. D., & Wu, J. C. . Chemically defined generation of human cardiomyocytes. Nature methods, 11(8), 855-860, (2014) Nakagawa, M., Taniguchi, Y., Senda, S., Takizawa, N., Ichisaka, T., Asano, K., Morizane, A., Doi, D., Takahashi, J., Nishizawa, M., Yoshida, Y., Toyoda, T., Osafune, K., Sekiguchi, K., & Yamanaka, S. . A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Scientific reports, 4(3594), 1-7, (2014) Takayama, K., Mitani, S., Nagamoto, Y., Sakurai, F., Tachibana, M., Taniguchi, Y., Sekiguchi, K., Mizuguchi, H.. Laminin 411 and 511 promote the cholangiocyte differentiation of human induced pluripotent stem cells. Biochemical and biophysical research communications, 474(1), 91-96, (2016) Musah, S., Dimitrakakis, N., Camacho, D. M., Church, G. M., Ingber, D. E.. Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a lomerulus Chip. Nature protocols, 13(7), 1662, (2018)
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