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Biotinylated Anti-Murine TNF-α



Catalog Number : 500-P64Gbt

Description

Produced from sera of goats pre-immunized with highly pure recombinant Murine TNF-α. Anti-Murine TNF-α specific antibody was purified by affinity chromatography and then biotinylated.

Additional Information

Immunogen
Recombinant Murine TNF-α (BioGems Catalog #315-01A)
 
Clone:
Polyclonal Goat
 
Format:
Biotin
 
Applications:
Direct Detection:
To detect Murine TNF-α by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine TNF-α.
Sandwich ELISA:
To detect Murine TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Murine TNF-α (61-124GP) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine TNF-α.
Western Blot:
To detect Murine TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity:
Mouse
 

Availability: In stock

Size Price Qty
25μg
$195.00
50μg
$250.00
1mg
$3,400.00