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Biotinylated Anti-Human PDGF-BB



Catalog Number : 500-P47bt

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB. Anti-Human PDGF-BB specific antibody was purified by affinity chromatography and then biotinylated.

Additional Information

Immunogen
Recombinant Human PDGF-BB (BioGems Catalog #100-14B)
 
Clone:
Polyclonal Rabbit
 
Format:
Biotin
 
Applications:
Direct Detection:
To detect hPDGF-BB by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hPDGF-BB.
Sandwich ELISA:
To detect hPDGF-BB by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Human PDGF-BB (60-267P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hPDGF-BB.
Western Blot:
To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity:
Human
 

Availability: In stock

Size Price Qty
25μg
$195.00
50μg
$250.00
1mg
$3,400.00