Rat TNF-α Pre-Coated ELISA Kit
description
The BioGems Rat TNF-α Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure TNF-α with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TNF-α. It contains E. coli-expressed recombinant TNF-α. The detection antibody is a biotinylated goat polyclonal antibody specific for TNF-α. The kit is analytically validated with ready to use reagents.
Product Details
|
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
|
Sensitivity:
|
<1pg/ml
|
|
Range:
|
15.6pg/ml-1000pg/ml(cellculturesupernates),7.8pg/ml-500pg/ml(serum,plasma)
|
|
Specificity:
|
Natural and recombinant Rat TNF-α
|
|
Uniprot:
|
P16599
|
|
Interference:
|
No detectable cross-reactivity with other relevant proteins
|
|
Storage Conditions:
|
Store the unopened product at 2 - 8 °C.
|
Components
Description | Quantity | Volume |
Anti-Rat TNF-α Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells |
Rat TNF-α Standard | 2 | 10 ng vial |
Rat TNF-α Biotinylated antibody (100x) | 1 | 130 µL |
Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL |
Sample Diluent | 1 | 30mL |
Antibody Diluent | 1 | 12mL |
Avidin-Biotin-Peroxidase Diluent | 1 | 12mL |
Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack |
Color Developing Reagent | 1 | 10mL |
Stop Solution | 1 | 10mL |
Plate Sealers | 4 | Each |
Reproducibility
| Intra-Assay | Inter-Assay | ||||
Sample ID | 1 | 2 | 3 | 1 | 2 | 3 |
n= | 16 | 16 | 16 | 24 | 24 | 24 |
Mean Measured Concentration (pg/mL) | 90 | 245 | 612 | 103 | 261 | 684 |
Standard Deviation | 4.77 | 7.6 | 20.81 | 6.9 | 15.4 | 44.5 |
Consistency (%CV) | 5.3 | 3.1 | 3.4 | 6.7 | 5.9 | 6.5 |