Murine VEGF Pre-Coated ELISA Kit

Grouped product items
Size Price Qty
1 Kit
$490.00
Catalog Number : BGK00731

description

VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.

The BioGems Murine VEGF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine VEGF with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for VEGF. It contains sf21-expressed recombinant Murine VEGF. The detection antibody is a biotinylated goat polyclonal antibody specific for VEGF. The kit is analytically validated with ready to use reagents.

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<2 pg/ml
Range:
15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Murine VEGF
Uniprot:
Q00731
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Murine VEGF Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine VEGF Standard

2

10 ng vial

Murine VEGF Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

126

341

580

212

475

624

Standard Deviation

4.41

13.64

28.42

10.6

30.4

46.8

Consistency (%CV)

3.5

4

4.9

5

6.4

7.5

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