Murine TGF-β1 Pre-Coated ELISA Kit

Grouped product items
Size Price Qty
1 Kit
$430.00
Catalog Number : BGK04202

description

The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of the biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β2 has been shown to exert suppressive effects on IL-2-dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development.

The BioGems Murine TGF- β1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine TGF- β1 with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for TGF- β1. It contains CHO-expressed recombinant Murine TGF- β1. The detection antibody is a biotinylated goat polyclonal antibody specific for TGF- β1. The kit is analytically validated with ready to use reagents.

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<2 pg/ml
Range:
15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Murine TGF-β1
Uniprot:
P04202
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Murine TGF- β1 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine TGF- β1 Standard

2

10 ng vial

Murine TGF- β1 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

114

240

483

125

248

515

Standard Deviation

5.47

12

17.9

6.75

15.4

30.4

Consistency (%CV)

4.8

5

3.7

5.4

6.2

5.9

© BioGems International, Inc. All rights reserved.