Murine TGF-β1 Pre-Coated ELISA Kit
description
The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The release of the biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β2 has been shown to exert suppressive effects on IL-2-dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development.
The BioGems Murine TGF- β1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine TGF- β1 with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for TGF- β1. It contains CHO-expressed recombinant Murine TGF- β1. The detection antibody is a biotinylated goat polyclonal antibody specific for TGF- β1. The kit is analytically validated with ready to use reagents.
Product Details
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
Sensitivity:
|
<2 pg/ml
|
Range:
|
15.6 pg/mL- 1,000 pg/mL
|
Specificity:
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Natural and recombinant Murine TGF-β1
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Uniprot:
|
P04202
|
Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description |
Quantity |
Volume |
Anti-Murine TGF- β1 Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Murine TGF- β1 Standard |
2 |
10 ng vial |
Murine TGF- β1 Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility
|
Intra-Assay |
Inter-Assay |
||||
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
114 |
240 |
483 |
125 |
248 |
515 |
Standard Deviation |
5.47 |
12 |
17.9 |
6.75 |
15.4 |
30.4 |
Consistency (%CV) |
4.8 |
5 |
3.7 |
5.4 |
6.2 |
5.9 |