Murine IL-2 Pre-Coated ELISA Kit
description
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.
The BioGems Murine IL-2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine IL-2 with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for IL-2. It contains E. coli-expressed recombinant Murine IL-2. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-2. The kit is analytically validated with ready to use reagents.
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<1 pg/ml
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Range:
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15.6 pg/mL- 1,000 pg/mL
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Specificity:
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Natural and recombinant Murine IL-2
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Uniprot:
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P04351
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
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Description |
Quantity |
Volume |
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Anti-Murine IL-2 Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
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Murine IL-2 Standard |
2 |
10 ng vial |
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Murine IL-2 Biotinylated antibody (100x) |
1 |
130 µL |
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Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
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Sample Diluent |
1 |
30mL |
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Antibody Diluent |
1 |
12mL |
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Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
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Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
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Color Developing Reagent |
1 |
10mL |
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Stop Solution |
1 |
10mL |
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Plate Sealers |
4 |
Each |
Reproducibility
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|
Intra-Assay |
Inter-Assay |
||||
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Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
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n= |
16 |
16 |
16 |
24 |
24 |
24 |
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Mean Measured Concentration (pg/mL) |
81 |
215 |
590 |
86 |
193 |
575 |
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Standard Deviation |
4.536 |
11.61 |
25.37 |
5.246 |
13.32 |
29.9 |
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Consistency (%CV) |
5.6 |
5.4 |
4.3 |
6.1 |
6.9 |
5.2 |