Human EGF Pre-Coated ELISA Kit
description
EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor).
The BioGems Human EGF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human EGF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for EGF. It contains E. coli-expressed recombinant Human EGF. The detection antibody is a biotinylated goat polyclonal antibody specific for EGF. The kit is analytically validated with ready to use reagents.
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<1 pg/ml
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Range:
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4.7 pg/mL- 300 pg/mL
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Specificity:
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Natural and recombinant Human EGF
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Uniprot:
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P01133
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
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Description |
Quantity |
Volume |
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Anti-Human EGF Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
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Human EGF Standard |
2 |
1 ng vial |
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Human EGF Biotinylated antibody (100x) |
1 |
130 µL |
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Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
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Sample Diluent |
1 |
30mL |
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Antibody Diluent |
1 |
12mL |
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Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
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Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
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Color Developing Reagent |
1 |
10mL |
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Stop Solution |
1 |
10mL |
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Plate Sealers |
4 |
Each |
Reproducibility
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Intra-Assay |
Inter-Assay |
||||
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Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
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n= |
16 |
16 |
16 |
24 |
24 |
24 |
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Mean Measured Concentration (pg/mL) |
42.1 |
105 |
197 |
45.2 |
112 |
227 |
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Standard Deviation |
1.98 |
5.67 |
6.3 |
3.21 |
5.71 |
13.17 |
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Consistency (%CV) |
4.70 |
5.4 |
3.19 |
7.10 |
5.09 |
5.80 |