Human ANG-2 Pre-Coated ELISA Kit
description
The BioGems Human ANG-2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure ANG-2 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for ANG-2. It contains NS0-expressed recombinant ANG-2. The detection antibody is a biotinylated goat polyclonal antibody specific for ANG-2. The kit is analytically validated with ready to use reagents.
Product Details
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
Sensitivity:
|
<10pg/ml
|
Range:
|
156pg/ml-10000pg/ml
|
Specificity:
|
Natural and recombinant Human ANG-2
|
Uniprot:
|
O15123
|
Interference:
|
No detectable cross-reactivity with other relevant proteins
|
Storage Conditions:
|
Store the unopened product at 2 - 8 °C.
|
Components
Description | Quantity | Volume |
Anti-Human ANG-2 Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells |
Human ANG-2 Standard | 2 | 10 ng vial |
Human ANG-2 Biotinylated antibody (100x) | 1 | 130 µL |
Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL |
Sample Diluent | 1 | 30mL |
Antibody Diluent | 1 | 12mL |
Avidin-Biotin-Peroxidase Diluent | 1 | 12mL |
Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack |
Color Developing Reagent | 1 | 10mL |
Stop Solution | 1 | 10mL |
Plate Sealers | 4 | Each |
Reproducibility
| Intra-Assay | Inter-Assay | ||||
Sample ID | 1 | 2 | 3 | 1 | 2 | 3 |
n= | 16 | 16 | 16 | 24 | 24 | 24 |
Mean Measured Concentration (pg/mL) | 1.08 | 3.49 | 7.01 | 1.05 | 3.32 | 6.67 |
Standard Deviation | 0.049 | 0.181 | 0.407 | 0.071 | 0.236 | 0.5 |
Consistency (%CV) | 4.5 | 5.2 | 5.8 | 6.8 | 7.1 | 7.5 |