Human ANG-2 Pre-Coated ELISA Kit

Grouped product items
Size Price Qty
1 Kit
$520.00
Catalog Number : BGK15123

description

ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich “coiled coil” N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.

The BioGems Human ANG-2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure ANG-2 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for ANG-2. It contains NS0-expressed recombinant ANG-2. The detection antibody is a biotinylated goat polyclonal antibody specific for ANG-2. The kit is analytically validated with ready to use reagents.

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
156pg/ml-10000pg/ml
Specificity:
Natural and recombinant Human ANG-2
Uniprot:
O15123
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human ANG-2 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human ANG-2 Standard

2

10 ng vial

Human ANG-2 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

1.08

3.49

7.01

1.05

3.32

6.67

Standard Deviation

0.049

0.181

0.407

0.071

0.236

0.5

Consistency (%CV)

4.5

5.2

5.8

6.8

7.1

7.5

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