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Anti-Rat TNF-α



Catalog Number : 500-P72

Description

Produced from sera of rabbits pre-immunized with highly pure recombinant Rat TNF-α. Anti-Rat TNF-α specific antibody was purified by affinity chromatography employing immobilized Rat TNF-α matrix.

Additional Information

Immunogen
Recombinant Rat TNF-α (BioGems Catalog #400-14)
 
Clone:
Polyclonal Rabbit
 
Format:
purified
 
Applications:
Immunohistochemistery:
This antibody stained colchicine injected rat brain (hippocampus CA1 region) tissue.  The primary antibody was incubated at 1.0 μg/ml overnight at 4˚C.  This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent.  Optimal concentrations and conditions may vary.   
Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat TNF-α (2.0 ng/ml), a concentration of 0.07-0.10 µg/ml of this antibody is required.
Sandwich ELISA:
To detect Rat TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Rat TNF-α (62-076BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-α.
Western Blot:
To detect Rat TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat TNF-α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity:
Rat
 

Availability: In stock

Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00