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Anti-Rat CD80 (B7-1) PE



Catalog Number : 02913-60

Description

The 3H5 monoclonal antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

The 3H5 antibody block the binding of CD80 to its ligands.

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Rat LPS-stimulated splenocytes were stained with PE 3H5 with autofluorescent cells in green.
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Additional Information

Clone:
3H5
Format:
PE
Applications:
FC
Reactivity:
Rat
Isotype:
Mouse IgG1, kappa
Research Interest: Innate Immunity, Adaptive Immunity
Cell Type: B Cells, Monocytes and Macrophages, T Cells, Dendritic Cells
Preparation:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:

Damoiseaux, J. G., Yagita, H., Okumura, K., & van Breda Vriesman, P. J. (1998). Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells. Journal of leukocyte biology64(6), 803-809.

Maeda, K., Sato, T., Azuma, M., Yagita, H., & Okumura, K. (1997). Characterization of rat CD80 and CD86 by molecular cloning and mAb.International immunology9(7), 993-1000.

Bluestone, J. A. (1995). New perspectives of CD28-B7-mediated T cell costimulation. Immunity2(6), 555-559.


Availability: In stock

Size Price Qty
100 ug
$200.00
200 ug
$320.00
Additional 3H5 Formats
Cat.No. Format Starting From
02913-60PE $ 200.00