Anti-Rat CD25 PE
description
The OX39 antibody specifically reacts with rat CD25, the 55 kDa low-affinity Interleukin-2 Receptor α chain (IL-2R α), expressed on early progenitors of T and B lineage, and on B and T cells. Together with CD122 (IL-2 Receptor β) and CD 132 (IL-2 Receptor γc, the common gamma chain), CD25 forms high-affinity receptor complexes for IL-2.
The OX39 antibody is reported to deplete CD25 cells in vivo and block some IL-2 mediated responses.
Additional Information
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Clone:
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OX39
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Format:
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PE
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Applications:
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FC
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Reactivity:
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Rat
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Isotype:
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Mouse IgG1, lambda
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| Research Interest: | Adaptive Immunity |
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| Cell Type: | B Cells, T Cells, T Regulatory Cells |
| Application: | FC |
| Clone: | OX39 |
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Preparation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Stephens, L. A., & Mason, D. (2000). CD25 is a marker for CD4+ thymocytes that prevent autoimmune diabetes in rats, but peripheral T cells with this function are found in both CD25+ and CD25− subpopulations. The Journal of Immunology, 165(6), 3105-3110. Vujanovic, N. L., Herberman, R. B., Maghazachi, A. A., & Hiserodt, J. C. (1988). Lymphokine-activated killer cells in rats. III. A simple method for the purification of large granular lymphocytes and their rapid expansion and conversion into lymphokine-activated killer cells. The Journal of experimental medicine, 167(1), 15-29. Stephens, L. A., Barclay, A. N., & Mason, D. (2004). Phenotypic characterization of regulatory CD4+ CD25+ T cells in rats. International immunology, 16(2), 365-375. |