Anti-Rat CD25 PE
The OX39 antibody specifically reacts with rat CD25, the 55 kDa low-affinity Interleukin-2 Receptor α chain (IL-2R α), expressed on early progenitors of T and B lineage, and on B and T cells. Together with CD122 (IL-2 Receptor β) and CD 132 (IL-2 Receptor γc, the common gamma chain), CD25 forms high-affinity receptor complexes for IL-2.
The OX39 antibody is reported to deplete CD25 cells in vivo and block some IL-2 mediated responses.
Mouse IgG1, lambda
|Research Interest:||Adaptive Immunity|
|Cell Type:||B Cells, T Cells, T Regulatory Cells|
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Stephens, L. A., & Mason, D. (2000). CD25 is a marker for CD4+ thymocytes that prevent autoimmune diabetes in rats, but peripheral T cells with this function are found in both CD25+ and CD25− subpopulations. The Journal of Immunology, 165(6), 3105-3110.
Vujanovic, N. L., Herberman, R. B., Maghazachi, A. A., & Hiserodt, J. C. (1988). Lymphokine-activated killer cells in rats. III. A simple method for the purification of large granular lymphocytes and their rapid expansion and conversion into lymphokine-activated killer cells. The Journal of experimental medicine, 167(1), 15-29.
Stephens, L. A., Barclay, A. N., & Mason, D. (2004). Phenotypic characterization of regulatory CD4+ CD25+ T cells in rats. International immunology, 16(2), 365-375.