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Anti-Murine Leptin



Catalog Number : 500-P68

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mLeptin. Anti-Murine Leptin specific antibody was purified by affinity chromatography employing immobilized mLeptin matrix.

Additional Information

Immunogen
Recombinant Murine Leptin (BioGems Catalog #450-31)
 
Clone:
Polyclonal Rabbit
 
Format:
purified
 
Applications:
Immunohistochemistery:
This antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine cecum and large intestine tissue. The primary antibody was incubated at 0.20 µg/mL overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary.
Sandwich ELISA:
To detect mLeptin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine Leptin (61-085BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.
Western Blot:
To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity:
Mouse
 

Availability: In stock

Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00