Creating Value for Life Science Research!


Get a Free Sample of this product!
shopping cart image

Anti-Murine JE/MCP-1



Catalog Number : 500-P113

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mJE(MCP-1). Anti-Murine JE(MCP-1) specific antibody was purified by affinity chromatography employing immobilized mJE (MCP-1) matrix.

Additional Information

Immunogen
Recombinant Murine JE/MCP-1 (CCL2) (BioGems Catalog #250-10)
 
Clone:
Polyclonal Rabbit
 
Format:
purified
 
Applications:
Immunohistochemistery:
This antibody stained colchicine injected mouse brain (including caudate putamen) tissue.  The primary antibody was incubated at 1.0 mg/ml overnight at 4˚C.  This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary.  
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mJE(MCP-1) (100 ng/ml), a concentration of 4.0-6.0 µg/ml of this antibody is required.
Sandwich ELISA:
To detect mJE(MCP-1) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine JE(MCP-1)(61-083BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).
Western Blot:
To detect mJE(MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE(MCP-1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity:
Mouse
 

Availability: In stock

Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00