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Anti-Murine IGF-I



Catalog Number : 500-P157G

Description

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIGF-I. Anti-Murine IGF-I specific antibody was purified by affinity chromatography employing immobilized mIGF-I matrix.

Additional Information

Immunogen
Recombinant Murine IGF-I (BioGems Catalog #250-19)
 
Clone:
Polyclonal Goat
 
Format:
purified
 
Applications:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Murine IGF-I (200 ng/ml), a concentration of 12.0 – 14.0 μg/ml of this antibody is required.
Sandwich ELISA:
To detect mIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine IGF-I (61-080BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIGF-I.
Western Blot:
To detect mIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity:
Mouse
 

Availability: In stock

Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00