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Anti-Mouse MHC Class II (I-A/I-E) BG Violet 450

Catalog Number : 86212-40


The M5/114.15.2 monoclonal antibody or (M5/114) is specific for the mouse major histocompatibility complex class II, specific for the I-A and I-E subregion-encoded glycoproteins (alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, but not the I-Af, I-Ak, nor I-As antigens). The antibody also reacts with cells from the H-2p or H-2r haplotypes mice.

The polymorphic determinant represented by the mouse major histocompatibility complex class II (MHC Class II) is expressed by the immune cells bearing the specified alloantigens, like T and B lymphocytes, monocytes, dendritic cells, and macrophages. The antibody doesn’t react with the NOD H-2g7 haplotype alloantigens.

M5/114 mAb inhibits I-A-restricted T cell responses of the Haplotypes H-2b, H-2d, H-2q, and H-2u.

BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

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C57BI/6 bone marrow cells were stained with BG Violet 450 M5/114.15.2 with relevant isotype control in Red.
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Additional Information

BG Violet 450
Rat IgG2b, kappa
Research Interest: Innate Immunity, Adaptive Immunity
Cell Type: B Cells, Monocytes and Macrophages, T Cells
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Bhattacharya, A. L. O. K., Dorf, M. E., & Springer, T. A. (1981). A shared alloantigenic determinant on Ia antigens encoded by the IA and IE subregions: evidence for I region gene duplication. The Journal of Immunology, 127(6), 2488-2495.

Mendiratta, S. K., Singh, N., Bal, V., & Rath, S. (1996). Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity.Immunology89(2), 238-244.

Unternaehrer, J. J., Chow, A., Pypaert, M., Inaba, K., & Mellman, I. (2007). The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proceedings of the National Academy of Sciences,104(1), 234-239.

Availability: In stock

Size Price Qty
25 μg
100 μg
Additional M5/114.15.2 Formats
Cat.No. Format Starting From
86212-40BG Violet 450 $ 80.00
86212-20Purified $ 100.00
86212-50FITC $ 50.00
86212-80APC $ 55.00
86212-60PE $ 60.00
86212-77PE-Cyanine7 $ 120.00