Anti-Mouse CD4 BG Violet 500
Additional RM4-5 Formats
| Cat.No. | Format | Starting From |
|---|---|---|
| 06122-60 | PE | $ 30.00 |
| 06122-70 | PerCP-Cyanine5.5 | $ 80.00 |
| 06122-77 | PE-Cyanine7 | $ 70.00 |
| 06122-40 | BG Violet 450 | $ 75.00 |
| 06122-50 | FITC | $ 30.00 |
| 06122-87 | APC-Cyanine7 | $ 65.00 |
| 06122-80 | APC | $ 65.00 |
| 06122-45 | BG Violet 500 | $ 85.00 |
description
The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells. CD4 binds to the major histocompatibility complex class II (MHC class II) and enhances T lymphocyte development and mature T cells functions. In T lymphocytes, CD4 binds to the cytoplasmic tail of enzyme tyrosine kinase (p56lck).
Binding of RM4-5 is blocked by the anti-mouse CD4 clone GK1.5.
BG Violet 500 conjugate is an alternative to the Pacific Orange and BD Horizon V500 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 500nm.
Additional Information
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Clone:
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RM4-5
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Format:
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BG Violet 500
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Applications:
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FC
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Reactivity:
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Mouse
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Isotype:
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Rat IgG2a, kappa
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| Research Interest: | Adaptive Immunity |
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| Cell Type: | T Cells, NK and NKT Cells, Macrophage / Monocyte |
| Application: | FC |
| Clone: | RM4-5 |
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Preparation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Shevach, E. M. (2000). Regulatory T cells in autoimmmunity*. Annual review of immunology, 18(1), 423-449. Wu, L., Scollay, R., Egerton, M., Pearse, M., Spangrude, G. J., & Shortman, K. (1991). CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus. Bliss, S. K., Bliss, S. P., Beiting, D. P., Alcaraz, A., & Appleton, J. A. (2007). IL-10 regulates movement of intestinally derived CD4+ T cells to the liver. The Journal of Immunology, 178(12), 7974-7983. |