Anti-Mouse CD3e PerCP-Cyanine5.5

Catalog Number : 05122-70

Description

The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa ε chain of the TCR complex. Together with the γ and δ subunits of CD3, the ε subunits are involved in the assembly, trafficking, and surface expression of T-cell receptor complex. CD3 is expressed on thymocytes, mature T cells, and natural killer T cells, and the ε chain enhances the antigen recognition.

The 145-2C11 antibody binds to the TCR complex and, depending on the conditions, initiates T cells activation, proliferation, and apoptosis. The soluble antibody seems to block lysis of target cells by antigen-specific cytotoxic T lymphocytes.

The 145-2C11 antibody does not cross-react with the rat leukocytes, and it is used as a phenotypic marker for mouse T lymphocytes.

C57Bl/6 splenocytes were stained with PerCP-Cy5.5 145-2C11 with relevant isotype control in Red.

Additional Information

Clone:	145-2C11
Format:	PerCP-Cyanine5.5
Applications:	FC
Reactivity:	Mouse
Isotype:	Armenian Hamster IgG
Research Interest:	Adaptive Immunity
Cell Type:	NK and NKT Cells, T Cells, Treg cells, Th17 cells
Preparation:	The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:	Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:	Leo, O., Foo, M., Sachs, D. H., Samelson, L. E., & Bluestone, J. A. (1987). Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proceedings of the National Academy of Sciences, 84(5), 1374-1378. Nakano, H., Yamazaki, T., Miyatake, S., Nozaki, N., Kikuchi, A., & Saito, T. (1996). Specific Interaction of Topoisomerase II and the CD3 Chain of the T Cell Receptor Complex. Journal of Biological Chemistry, 271(11), 6483-6489. Salmond, R. J., Filby, A., Pirinen, N., Magee, A. I., & Zamoyska, R. (2011). Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas. Blood, 117(1), 108-117.

Availability: In stock

Size	Price	Qty
25 μg	$70.00
100 μg	$205.00

Additional 145-2C11 Formats

Cat.No.	Format	Starting From
05122-60	PE	$ 30.00
05122-65	PE-Cyanine5	$ 65.00
05122-70	PerCP-Cyanine5.5	$ 70.00
05122-77	PE-Cyanine7	$ 70.00
05122-20	Purified	$ 65.00
05122-25	SAFIRE Purified	$ 105.00
05122-50	FITC	$ 25.00
05122-30	Biotin	$ 35.00
05122-80	APC	$ 55.00