Anti-Mouse CD3e Biotin
Additional 145-2C11 Formats
Cat.No. | Format | Starting From |
---|---|---|
05122-60 | PE | $ 30.00 |
05122-65 | PE-Cyanine5 | $ 65.00 |
05122-70 | PerCP-Cyanine5.5 | $ 70.00 |
05122-77 | PE-Cyanine7 | $ 70.00 |
05122-20 | Purified | $ 65.00 |
05122-25 | SAFIRE Purified | $ 105.00 |
05122-50 | FITC | $ 25.00 |
05122-30 | Biotin | $ 35.00 |
05122-80 | APC | $ 55.00 |
description
The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa ε chain of the TCR complex. Together with the γ and δ subunits of CD3, the ε subunits are involved in the assembly, trafficking, and surface expression of T-cell receptor complex. CD3 is expressed on thymocytes, mature T cells, and natural killer T cells, and the ε chain enhances the antigen recognition.
The 145-2C11 antibody binds to the TCR complex and, depending on the conditions, initiates T cells activation, proliferation, and apoptosis. The soluble antibody seems to block lysis of target cells by antigen-specific cytotoxic T lymphocytes.
The 145-2C11 antibody does not cross-react with the rat leukocytes, and it is used as a phenotypic marker for mouse T lymphocytes.
Additional Information
Clone:
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145-2C11
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Format:
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Biotin
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Applications:
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FC
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Reactivity:
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Mouse
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Isotype:
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Armenian Hamster IgG
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Research Interest: | Adaptive Immunity |
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Cell Type: | NK and NKT Cells, T Cells, T Regulatory Cells, Th17 cells |
Application: | FC |
Clone: | 145-2C11 |
Preparation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Leo, O., Foo, M., Sachs, D. H., Samelson, L. E., & Bluestone, J. A. (1987). Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proceedings of the National Academy of Sciences, 84(5), 1374-1378. Nakano, H., Yamazaki, T., Miyatake, S., Nozaki, N., Kikuchi, A., & Saito, T. (1996). Specific Interaction of Topoisomerase II and the CD3 Chain of the T Cell Receptor Complex. Journal of Biological Chemistry, 271(11), 6483-6489. Salmond, R. J., Filby, A., Pirinen, N., Magee, A. I., & Zamoyska, R. (2011). Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas. Blood, 117(1), 108-117. |