Anti-Mouse CD25 APC

Catalog Number : 07312-80

Description

The PC61.5 antibody specifically reacts with mouse CD25, the 55 kDa low-affinity Interleukin-2 Receptor α chain (IL-2R α), expressed on early progenitors of T and B lineage, and on B and T cells. Together with CD122 (IL-2 Receptor β) and CD 132 (IL-2 Receptor γc, the common gamma chain), CD25 forms high-affinity receptor complexes for IL-2. Resting B and T cells and natural killer cells do not express IL-2Rα. Cd25 is also expressed on the dendritic cells, and it enhances lymphocyte differentiation and activation.

The PC61.5 antibody blocks the binding of IL-2 to both high-affinity and low-affinity receptors.

C57Bl/6 splenocytes were stained with APC PC61.5 and FITC GK1.5.

Additional Information

Clone	PC61.5
Format:	APC
Applications	FC
Reactivity	Mouse
Isotype:	Rat IgG1, lambda
Research Interest:	Adaptive Immunity
Cell Type:	B Cells, T Cells, Treg cells
Preperation:	The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:	Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:	Hayashi, T., Hasegawa, K., & Adachi, C. (2005). Elimination of CD4+ CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZB× NZW F1 mice. International journal of experimental pathology, 86(5), 289-296. Lowenthal, J. W., Tougne, C., MacDonald, H. R., Smith, K. A., & Nabholz, M. (1985). Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone. The Journal of Immunology, 134(2), 931-939. Huang, B., Zhao, J., Shen, S., Li, H., He, K. L., Shen, G. X., ... & Feng, Z. H. (2007). Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling. Cancer Research, 67(9), 4346-4352.

Availability: In stock

Size	Price	Qty
25 μg	$75.00
100 μg	$225.00

Additional PC61.5 Formats

Cat.No.	Format	Starting From
07312-60	PE	$ 60.00
07312-70	PerCP-Cyanine5.5	$ 90.00
07312-77	PE-Cyanine7	$ 70.00
07312-40	BG Violet 450	$ 90.00
07312-20	Purified	$ 65.00
07312-25	SAFIRE Purified	$ 120.00
07312-80	APC	$ 75.00
07312-50	FITC	$ 55.00