Anti-Mouse CD19 BG Violet 450
description
The 1D3 monoclonal antibody specifically reacts with mouse CD19, a 95 kDa transmembrane glycoprotein, a member of the Ig superfamily and a B cell-lineage differentiation antigen expressed by all the B lymphocyte development stages, except for the terminally differentiated plasma cells.
CD19 associates with CD21, CD81 and MHC class II to form a multi-molecular complex that initiates the mature B lymphocyte activation by interaction with the B-cell receptors. CD 19 enhances the B cell proliferation, development and activation, and the maturation of memory B cells. In CD19-deficient mice, the generation and maturation of B lymphocytes in the bone marrow and periphery are affected.
BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.
Additional Information
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Clone:
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1D3
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Format:
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BG Violet 450
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Applications:
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FC
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Reactivity:
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Mouse
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Isotype:
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Rat IgG2a, kappa
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| Research Interest: | Adaptive Immunity |
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| Cell Type: | B Cells |
| Application: | FC |
| Clone: | 1D3 |
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Preparation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Cherukuri, A., Cheng, P. C., & Pierce, S. K. (2001). The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens.The Journal of Immunology, 167(1), 163-172. Krop, I., De Fougerolles, A. R., Hardy, R. R., Allison, M., Schlissel, M. S., & Fearon, D. T. (1996). Self-renewal of B-1 lymphocytes is dependent on CD19.European journal of immunology, 26(1), 238-242. Engel, P., Zhou, L. J., Ord, D. C., Sato, S., Koller, B., & Tedder, T. F. (1995). Abnormal B lymphocyte delevopment, activation, and differentiation in mice that lack or overexpress the CD19 signal transduction molecule. Immunity, 3(1), 39-50. Sato, S., Jansen, P. J., & Tedder, T. F. (1997). CD19 and CD22 expression reciprocally regulates tyrosine phosphorylation of Vav protein during B lymphocyte signaling. Proceedings of the National Academy of Sciences,94(24), 13158-13162. |