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Anti-Human WNT-3a



Catalog Number : 500-P251

Description

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Anti-Human WNT-3a specific antibody was purified by affinity chromatography employing immobilized hWNT-3a matrix.

Additional Information

Immunogen
(BioGems Catalog #)
 
Clone:
Polyclonal Rabbit
 
Format:
purified
 
Applications:
Immunohistochemistery:
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 μg/ml - 0.25 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Sandwich ELISA:
To detect hWNT-3a by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human WNT-3a (60-403BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.
Western Blot:
To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity:
Human
 

Availability: In stock

Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00