Catalog Number : 500-M88
Produced in BALB/c mice using highly pure (>98%) recombinant human VEGF165 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A chromatography.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human VEGF (BioGems Catalog #100-20)
To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10 ng/ml), a concentration of ≤ 0.2 μg/ml of this antibody is required.
In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant human VEGF165 when used with BioGems' biotinylated antigen affinity purified anti-human VEGF165 (60-342BT) as the detection antibody at a concentration of approximately 0.25-1.0 μg/ml.
To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.