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Anti-Human TNF-α



Catalog Number : 500-P31A

Description

Produced from sera of rabbits pre-immunized with highly pure recombinant Human TNF-α. Anti-Human TNF-α specific antibody was purified by affinity chromatography employing immobilized Human TNF-α matrix.

Additional Information

Immunogen
Recombinant Human TNF-α (BioGems Catalog #300-01A)
 
Clone:
Polyclonal Rabbit
 
Format:
purified
 
Applications:
Immunohistochemistery:
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.5 μg/ml-1.0 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
*Additional Immunostaining data available. Please contact Tech Support for information.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-α (0.5 ng/ml), a concentration of 0.08- 0.10 µg/ml of this antibody is required.
Sandwich ELISA:
To detect Human TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human TNF-α (60-325BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-α.
Western Blot:
To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
 
Reactivity:
Human
 

Availability: In stock

Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00