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Anti-Human TNF-β



Catalog Number : 500-P31B

Description

Produced from sera of rabbits pre-immunized with highly pure recombinant Human TNF-β. Anti-Human TNF-β specific antibody was purified by affinity chromatography employing immobilized Human TNF-β matrix.

Additional Information

Immunogen
Recombinant Human TNF-β (E.coli-derived) (BioGems Catalog #300-01B)
 
Clone:
Polyclonal Rabbit
 
Format:
purified
 
Applications:
Immunohistochemistery:
This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant carcinoma.  The recommended concentration is 0.50 µg/mL with an overnight incubation at 4˚C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary.  
 Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. 
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-β (0.5 ng/ml), a concentration of < 0.1 μg/ml of this antibody is required.
Sandwich ELISA:
To detect Human TNF-β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human TNF-β (60-325BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-β.
Western Blot:
To detect Human TNF-β by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-β is 1.5-3.0 ng/lane, under either reducing non-reducing conditions.
 
Reactivity:
Human
 

Availability: In stock

Size Price Qty
50μg
$195.00
100μg
$250.00
1mg
$1,700.00